The P2Y6 receptor is a purinoceptor member of P2receptor family as well as responsible for microglial phagocytosis in the CNS.18 To directly examine the inhibitory effect of MRS2578 on microglia in vitro, we cultured primary microglia using LPS and UDP stimulation to mimic the damage after ischemic stroke in vivo.18 After 12 hours LPS and UDP (100 μmol/L) stimulation, microglia were incubated with fluorescent latex beads for 2 hours. Here, P2RY6 is linked to ischemic stroke.