To determine the impact of these modifications on IGF2BP1 function, we transduced CD34+ cells from four healthy adult donors with the parental SIiP virus or versions that included the 2A-puromcyin (SI2AP) or 2A-ZsGreen (SI2AZG) cassette at equivalent multiplicities of infection (MOIs) (Figure S3B). This evidence concerns the gene CD34 and infection.