Thus, to test whether PGE2 production and PD-L1 upregulation were induced in bovine PBMCs under TLR2 stimulation, PBMCs from uninfected cattle were incubated with a TLR2/6 agonist (FSL-1, 28), which is a synthetic lipoprotein of M. salivarium, which been implicated in eye and ear disorders, oral infection, septic arthritis, and periodontal disease in human (28), and PGE2 production in culture supernatants and PD-L1 expression on monocytes were analyzed. The gene discussed is TLR2; the disease is periodontal disorder.