Compared to untreated control cells, treatment (PGCCs with their daughter cells) had higher tumorigenicity and metastasis, evident from the tumor growth speed, size and the time of tumorigenesis (Figures 5A,B and Table 1), while these high tumorigenic and metastatic abilities significantly declined in the group of si-treatment (PGCCs with their daughter cells after S100A4 knockdown) (Figures 5A,B and Table 1). This evidence concerns the gene S100A4 and neoplasm.