To further confirm all the above in vitro results showing that knockdown of hsa_circ_0088732 suppressed glioma cell proliferation and metastasis by regulating the miR-661/RAB3D axis, we generated four groups of LN229 cells transfected with (1) transfection reagent (Blank), (2) NC, (3) hsa_circ_0088732 siRNA or (4) hsa_circ_0088732 overexpression plasmids. The gene discussed is RAB3D; the disease is central nervous system cancer.