Glypican-1 (GPC-1) is a heparan sulfate proteoglycan (HSPG) that binds to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) anchor.17,18 We identified GPC-1 as an antigen for oesophageal squamous cell carcinoma (ESCC) using quantitative proteomics targeting the cell surface membrane protein. This evidence concerns the gene GPC1 and esophageal squamous cell carcinoma.