For that purpose, we transfected siRNA sequences against vimentin (Vim Si1 and Vim Si2) in well-known EMT+ MDA-MB-231 human breast tumor cells and in human cell systems previously reported to be inducible for EMT by EGF (MDA-MB-468, PMC42-LA) or TGF-β1 (Α549). Here, TGFB1 is linked to breast neoplasm.