Overexpression of TNFAIP8 combined with OA treatment also induced the expression of LC3β I/II in all three indicated HCC cell lines and quantitation of ORO-based steatosis (lipid content) suggests that TNFAIP8 expression in HCC cells increased oleic acid uptake/accumulation by ~1.5–2.1-fold in HCC cells (Fig. 6g, h). The gene discussed is TNFAIP8; the disease is hepatocellular carcinoma.