On the other hand, no significant changes in expression of TNFAIP8 mRNA/protein and autophagy markers (Beclin-1 and LC3β I/II) were observed in liver tissues from HFD compared with chow diet-fed mice (Supplementary Fig. 6b–d) suggesting hepatic steatosis induced by HFD in mice is not associated with TNFAIP8. This evidence concerns the gene BECN1 and Hepatic steatosis.