Moreover, inactivation of autophagy by 3-MA and treatment with anti-liver cancer drugs sorafenib or regorafenib in HCC cells significantly prevented TNFAIP8-mediated cell survival (Fig. 5e, f), suggesting that TNFAIP8-mediated induction of autophagy is required for cell survival and drug resistance in HCC cells. This evidence concerns the gene TNFAIP8 and liver cancer.