Given the observation that a high-fat diet was associated with fewer leptin-responsive neurons and astrocytes in the DVC (Fig. 2) and an attenuated hindbrain leptin response in vivo (Fig. 3), we next investigated maladaptive changes in the neuro-glia landscape of the DVC in response to obesity and, within the same rats, compared these changes to those observed in the arcuate nucleus of the hypothalamus. Here, LEP is linked to obesity disorder.