Our RNA-seq analysis shows that the genes associated with vitelline membrane and eggshell assembly – such as Cp36, nudel, Femcoat, dec-1 and Cp7Fc – are downregulated upon ZIKV infection, while others have reported that mutation in Cp36 and dec-1 results in female sterility (Hawley and Waring, 1988; Mauzy-Melitz and Waring, 2003). This evidence concerns the gene NDEL1 and Zika virus infectious disease.