The Vitamin D concentrations of 0.0002 μM, 0.002 μM, 0.02 μM, and 0.2 were effective in significantly regulating all targets examined in a melanoma cell line, except for p53 promoter activity that was upregulated by Vitamin D concentrations of 0.02 μM and 0.2 μM. Here, TP53 is linked to melanoma.