In this study, whole-genome sequencing revealed that the DNA in L-EVs reflects the genetic aberrations of the cell of origin, including copy number variations (CNV) of genes frequently altered in metastatic prostate cancer, such as MYC, AKT1, focal adhesion kinase [FAK, also known as protein tyrosine kinase 2 (PTK2)], KLF10, and PTEN. This evidence concerns the gene PTK2 and metastatic prostate carcinoma.