As a result of this study, regarding the evaluation of Nit and MTAP against the first strain (S1), which was isolated from the blood in a patient with sepsis, Nit showed a complete eradication with zero CFU in the canal lumen as well as the dentinal chips, from the lowest concentration (6.25 mg/mL) onwards, while MTAP could not eradicate this strain from the root canal lumen at the lower concentrations, neither in 6.25 nor at 12.5 mg/mL, but it could achieve a complete eradication with zero CFU at 25 mg/mL upwards. This evidence concerns the gene MTAP and Sepsis.