Although there were also studies to confirm the roles of Gli2 (Glioma-associated oncogene family member 2) [57], PDK1 (pyruvate dehydrogenase kinase) [58], NDUFAF1 (NADH dehydrogenase 1 alpha subcomplex assembly factor 1) [59], SLAMF7 (SLAM family member 7) [60], and CH25H (cholesterol 25-hydroxylase) [61], their conclusions seemed to be opposite with our study, which may be attributed to the differences in cancer type or sample size and thus further validation experiments are needed. This evidence concerns the gene CH25H and cancer.