NAMPT and lung carcinoma: To test the role of the NAD+/NADH ratio in mdivi-1-mediated inhibition of oxidative metabolism, we treated H460 cells with NMN (500 μM), to increase the cellular NAD+ level,47 and FK866 (100 nM), an inhibitor of nicotinamide phosphoribosyl transferase, to decrease the cellular NAD+ level.48 Neither NMN or FK866 treatment altered the levels of m + 2 citrate, α-KG and malate in H460 cells (Fig. 5b), suggesting that alteration of NAD+/NADH ratio had no effect on oxidative metabolism in H460 lung cancer cells.