As the alteration of TLR4/MyD88 expression is commonly linked to the downstream regulation of STAT3 and Sp1 (J. X. Chen et al., 2017; Xiao et al., 2017), we examined if reactivation of TLR4 by its agonist LPS could reverse geniposide‐induced inhibition of STAT3 and Sp1 in HCC cells. This evidence concerns the gene TLR4 and hepatocellular carcinoma.