We assayed these 5 compounds (the mTOR inhibitor Everolimus, the Chk1 inhibitor AZD-7762, the Ikk inhibitor IMD-0354 and the Plk1 inhibitors Volasertib and Rigosertib) in our Matrigel-based 3D assay and found that all inhibited cyst growth (Supplementary Table 5). Here, CHEK1 is linked to cyst.