To investigate the effect of DJ-1 on ferroptosis, we established several stable DJ-1 knockdown (KD) cancer cell lines (H1299, PANC1, A549, H292, and H838) by using two specific short hairpin RNA (shRNAs) against DJ-1 (referred to as DJ-1 KD #1 and #2; Fig. 1a, Supplementary Fig. 1a). Here, PARK7 is linked to cancer.