To further confirm our hypothesis that anti-miR-153 may protect the heart against myocardial infarct, we collected serum samples from four groups of rat (sham, I/R, I/R + miR-NC, and I/R + anti-miR-153) and the expression levels of four proteins (BNP, Brain natriuretic peptide; CK-MB, creatine kinase-MB; AST, aspartate aminotransferase; LDH, lactate dehydrogenase) as the indicators for ischemia/reperfusion injury were measured [28, 29]. This evidence concerns the gene NPPB and myocardial infarction.