At the same time, the HFD groups developed lipid metabolism disorders accompanied by liver lipid accumulation (higher TC and TG concentrations), hepatic function damage (higher serum ALT and AST levels), oxidative stress, and the resulting lipid peroxidation (a reduction of Gpx activity and increase of MDA content), which resembles the clinical symptoms of human NAFLD. Here, GPT is linked to metabolic dysfunction-associated steatotic liver disease.