Overall, while inhibitors of Ras and RhoA showed differential effects in the CTD Ser2 and Ser5 phosphorylation status depending on the cancer cell lines, inhibitors of Rac1 and Cdc42 more consistently reduced Ser2P and Ser5P levels in both cell lines without greatly affecting Ser7P and total RPB1 protein accumulation. The gene discussed is RHOA; the disease is cancer.