FLII and neoplasm: To establish the orthotopic tumour model, around 100 red, (td)Tomato fluorescent CRMM1 or CRMM2 cells were injected RO at 2 dpf into tg(Fli:GFP/Casper), endothelial reporter transgenic zebrafish with green fluorescent vasculature and examined by fluorescent microscopy at day 1 and 4 after engraftment (Figure 6A).