Since MCD feeding was shown to interfere with BA metabolism,39 recently found to be involved in progression of NASH,20 expression of key determinants of BA homeostasis, such as Cyp27a1, Cyp2c70 (alternative BA synthesis pathway) and Cyp7a1, Cyp8b1 (classic BA synthesis pathway), serum levels of 7‐hydroxy‐4‐cholesten‐3‐one (C4) as marker of BA synthesis as well as expression of FXR (main BA sensor) and its downstream target Shp were investigated in our mouse model. This evidence concerns the gene CYP8B1 and metabolic dysfunction-associated steatohepatitis.