Interestingly, in PC-3 and DU145 cells, we also observed that FER1L4 overexpression decreased the phosphorylation of AKT protein which was reversed towards FBXW7 silencing (Fig. 7c, d), indicating PI3K/AKT signaling was also regulated by FER1L4/FBXW7 in prostate cancer cells. This evidence concerns the gene AKT1 and Familial prostate cancer.