Importantly, immunofluorescence analysis showed that CRC-derived EVs were efficiently taken up by α-smooth muscle actin + hepatic stellate cells (α-SMA + hStCs), myofibroblasts, S100A4 + fibroblasts, and F4/80+ macrophage cells, but not by CD31 + endothelial cells in the liver and lung (Fig. 3b). This evidence concerns the gene S100A4 and colorectal carcinoma.