Using above lung and liver metastatic tissues from CRC patients, we detected the α-SMA (characteric marker for myofibroblastic CAFs) expression by IHC staining analysis, and the results showed high density of α-SMA + myofibroblasts around the metastatic tumors (Supplementary Fig. 16), implying the important function of α-SMA + myofibroblasts on metastatic CRC development. Here, ACTA1 is linked to metastatic neoplasm.