AKT1 and neoplasm: We examined this in two ways: (1) by ELISA quantitation of PIP2 levels in ECs obtained from tumors and lungs collected at the termination of 14-day experiments from tumor-allografted animals, and (2) by measuring active ERK1/2 and AKT in ECs from immunostained histological sections from tumors and livers of these same mice (Fig. 4b).