TGFB1 and gastric cancer: DAPI and F-actin staining confirmed that the HMrSV5 cells exhibited a more ductile morphology (an indicator of more invasive behavior) in the presence of metastatic gastric cancer cell supernatants, while the invasive behavior of HMrSV5 cells in the presence of peritoneal metastatic gastric cancer cell supernatants were greatly rescued by TGF-β inhibitor (Figure 4B).