To test whether Nec-1 would alleviate kidney damage caused Ang II, an animal model with properties of progressive renal tubulointerstitial fibrosis and CKD was established through continuous infusion of AngII (1.5μg/kg/min) using osmotic minipumps implanted subcutaneously in the backs of C57BL/6 mice that had undergone uninephrectomy. The gene discussed is PCSK1; the disease is chronic kidney disease.