INS and Insulin resistance: Further, the hyperinsulinemic-euglycemic clamp technique, the “gold standard” for evaluating insulin sensitivity (Lalia et al., 2015), confirmed the establishment of insulin resistance in C57BL/6J mice fed an HFD for 12 weeks, including excessive HGP, and establishment of a glucose uptake barrier in fat and muscle tissues, both of which were mitigated by AMC treatment, which significantly reduced insulin resistance in these peripheral target tissues.