In addition, a significant increase in Tlr4 expression was detected in the dorsal raphe region in WT and KO mice fed with WD compared to groups fed with CD (p = 0.042 and p = 0.040, respectively, Tukey’s test) but not in HET mice, and in the prefrontal cortex in the KO-WD group compared to the WT-WD group (p = 0.0403, Tukey’s test). Here, TLR4 is linked to Wilson disease.