To determine whether OGE could affect cell cycle related checkpoint proteins, we further examined the expressions of CDK4, CDK2, p-ERK1/2, and PFKFB3 by immunoblotting in HCC cells after treatment with different concentrations (0, 400, and 600 μg/mL) of OGE for 24 h. This evidence concerns the gene MAPK3 and hepatocellular carcinoma.