ACAD9 and neoplasm: HCT116 cells were subcutaneously implanted and, when formed, the tumour mass injected with a miR-27a inhibitor or a scrambled control as we previously reported.26 HK1, HK2, DLAT, CPT1A and ACAD9, representative of the overall mitochondrial activity, displayed higher expression in tumours injected with a miR-27a anti-sense than those injected with a scrambled control (Supplementary Fig. 3G).