An immediate application of EADSA-μLESA-LC-MS could be to assess the changes in 24S-HC, 24S,25-EC, desmosterol, and cholesterol in pathological niches in animal models of HD, and human postmortem tissues, and to monitor the production and distribution of 24S-HC in brain as a result of adeno-associated virus-mediated delivery of CYP46A1 into mouse striatum. This evidence concerns the gene CYP46A1 and Huntington disease.