KCNA1 and Bilateral tonic-clonic seizure: To investigate the ability of Kcna1-dCas9 to treat chronic temporal lobe epilepsy we analysed the frequency of generalized tonic-clonic seizures (Racine stage 5) in each animal before and after doxycycline administration using continuous video-EEG recordings (Fig. 3D–F, Supplementary Fig. 7 and Supplementary Video 2).