Although there exists limitations in this study including the evidence for that the miR-0308-3p overexpressing HCC are less aggressive and patients have better survival rate remains further investigated, as well as for such large scale screening of miRNA, the cut off as p < 0.01 may not appropriate, we found a novel miRNA, miR-0308-3p, that inhibited cancer cell proliferation and induced G1/S arrest via targeting double CDK6/Cyclin D1 genes. Here, CDK6 is linked to hepatocellular carcinoma.