In order to determine the commonalities between ciliating and non-ciliating cancer cells we first looked to see if the two key observations from ciliating cells, i.e. increased pPRK1/pPRK2 levels upon serum withdrawal and reduced spheroid growth upon PRK1 and/or PRK2 depletion, are also observed in TNBC MET1 cells. This evidence concerns the gene PKN1 and cancer.