Considering the possibility that PDIA3P1 may activate the NF-κB pathway and promote glioma MES transition through increasing RELA expression, we knocked down RELA using small interfering RNAs (siRNAs) in U87MG, A172, and U251 cells and determined the protein levels of CD44, β-catenin, N-cadherin, Vimentin, and MMP14. The gene discussed is NFKB1; the disease is central nervous system cancer.