To investigate how GNAS regulates IL-6 expression in HCC cells, phosphorylated NF-κB subunit p65 (p-p65) was detected and we found that GNAS knockdown did not obviously affect LPS stimulation-induced phosphorylation of p65 in HCC cells (Fig. 3a). The gene discussed is NFKB1; the disease is hepatocellular carcinoma.