(iii) Cla is less effective in cells expressing hERG1-K525C and in cells treated with E4031; both these conditions tend to increase the steady-state fraction of open channels, although for different reasons; (iv) the effect of Cla is facilitated by expression of channels that have a low steady-state probability of opening at the normal Vrest of CRC cells, such as hERG1-WT and hERG1-R531C. This evidence concerns the gene KCNH2 and colorectal carcinoma.