To evaluate the antitumor activity of Pmel-1 x SLAMF6 -/- cells, we assessed adoptive cell transfer (ACT) of 7 day pre-activated gp100:25–33-specific, Pmel-1 or Pmel-1 x SLAMF6 -/- CD8+ T cells, transferred into mice bearing palpable B16-F10/mhgp100 melanoma in their back skin, followed by a 2-day course of intraperitoneal IL-2 (Figure 4G–J). This evidence concerns the gene PMEL and melanoma.