We observed more than 20 pathways that were significantly affected between Sirt2-expressing and Sirt2-deficient cells (Fig. 3); these pathways include MAPK (P = 6.84 × 10− 05), TNF (P = 1.08 × 10− 04), fluid shear stress and atherosclerosis (P = 1.19 × 10− 04), proteoglycans in cancer (P = 2.20 × 10− 04), and cytokine–cytokine receptor interaction (P = 4.54 × 10− 04). The gene discussed is TNF; the disease is cancer.