The number of Melan-A-positive epidermal melanocytes was increased in the lesional skin of Riehl’s melanosis (melanocytes per 1 mm length of rete ridge (MC/1R), 17.81 ± 4.35) compared to perilesional normal-appearing skin (MC/1R, 12.09 ± 4.07; p = 0.01) and healthy controls (MC/1R, 10.45 ± 5.05; p = 0.01; Table 2). This evidence concerns the gene MLANA and freckles.