Quantification of infiltrating T cells (cells positive for CD3 or CD8 expression) on full tumor tissue sections, showed that preoperative radiation therapy was associated with a significant reduction in CD8 positive T cell density in the central tumor (CD8: p = 0.017) as well as reduced CD3, CD8 density and PD-1 expression level on TILs at the invasive margin (CD3: p = 0.006, CD8: p = 0.001, PD-1: p = 0.005). This evidence concerns the gene CD8A and neoplasm.