To verify whether gga-miR-221-5p interacts with ELOVL6 and SQLE genes, we constructed Vector-Luc-ELOVL6-WT 3′UTR, Vector-Luc-ELOVL6-Mut 3′UTR, Vector-Luc-SQLE-WT 3′UTR, and Vector-Luc-SQLE-Mut 3′UTR for the verification of the interaction between gga-miR-221-5p and the two potential target genes (Figure 6A,B), and the dual luciferase reporter assay was conducted in LMH chicken hepatoma cells. Here, ELOVL6 is linked to hepatocellular carcinoma.