For example, all four IDH1-wt glioblastoma GSCs with BRAF hotspot mutations (V600E and D594H) were classified into the E&F-independent group (Figure 3 and Table S4), although BRAF alterations are rarely observed in adult HGG or other diffusely infiltrating gliomas (2–5%) [22]. This evidence concerns the gene IDH1 and glioblastoma.