TP53 and central nervous system cancer: In one study comparing the results between IHC and PCR to detect only TP53 missense mutations in 61 low-grade gliomas, IHC was reported to have a sensitivity of 92% and specificity of 79% when comparing to PCR.20 When removing all 30 nonmissense mutated cases from our analysis, the calculated sensitivity and specificity values are 91.5% and 77.4% when comparing IHC to FM, which are similar to the study previously mentioned.