The RAS and MAPK signaling pathway showed similar activity between AK-organoids and CRC samples (P = 0.11 and P = 0.33, Figures S5C,D), suggesting the combination of APC and KRAS mutations enabled the activity of RAS and MAPK signaling pathway to reach the physiological state of CRCs. The gene discussed is KRAS; the disease is colorectal carcinoma.