To examine the mechanism underlying the suppression of GC cell proliferation and the induction of apoptosis by PAC, GC cells were transfected with a Notch1 (NTM) and/or Notch2 (NTM) overexpression plasmid, with contrasting expression profiles for proteins in the Notch/PTEN/AKT axis. This evidence concerns the gene NOTCH1 and gastric cancer.