COL4A5 and Alport syndrome: Hemizygous mutations of the COL4A5 gene [(c.4298G > T, p.G1433V), (c.1949-1G > A, IVS25), and (c.573_c.574insG, p. G192Gfs*24)] in fibroblasts and iPSCs of Alport syndrome patients were confirmed by genomic PCR and Sanger sequencing (Figure 1F).