By contrast, TNF-α+ cells co-expressing CD14 (cluster 1, N = 301; cluster 3, N = 310; and cluster 5, N = 68; Figure 3D, lower panel; Supplementary Table 3) mostly originated from antigen stimulated samples of LTBI adolescents and TB adults while they were almost absent in samples from antigen unstimulated and stimulated TB adolescents and LTBI adults (Supplementary Figure 1). This evidence concerns the gene TNF and tuberculosis.